Journal: bioRxiv

Article Title: Acyl Carrier Protein is Essential for Apicoplast Biogenesis in Malaria Parasites Independent of Fatty Acid Synthesis

doi: 10.64898/2026.03.29.713301

Figure Lengend Snippet: Integration PCRs for deletion or modification of the apicoplast ACP gene. ( A ) Genotyping PCR for successful deletion of the apicoplast ACP gene (Pf3D7_0208500) in polyclonal PfMev parasites showing selective amplification of sequence at the 5’ and 3’ ends of the drug-resistance marker (Δ5’ and Δ3’) in knockout parasites but not in parental PfMev parasites and exclusive amplification of sequence at the 5’ and 3’ ends of the ACP CDS in WT but not ΔACP parasites. Genotype PCR analysis confirming on-target integration of the 3’ aptamer/TetR-DOZI cassette at the apicoplast ACP locus in polyclonal PfMev ( B ) or clonal Dd2 parasites ( C ) and the absence of the unmodified wildtype (WT) locus, with parental parasites serving as negative control for integration.

Article Snippet: Purified protein was then injected into a rabbit for polyclonal antibody production by Cocalico Biologicals Inc ( https://cocalicobiologicals.com ) following their standard protocol.

Techniques: Modification, Amplification, Sequencing, Marker, Knock-Out, Negative Control